Observe the Effect of Helicobacter Pylori on Proliferation and Apoptosis of Gastric Epithelial Cells and Gastric Cancer Cells under Microscope

  • Qingfang Shi
Keywords: Microscope, Helicobacter pylori, Gastric Epithelial Cells, Cell Proliferation and Apoptosis

Abstract

Helicobacter pylori is a micro-anaerobic, spiral-shaped bacterium with very strict growth requirements. It is the
only type of microorganism known to be able to survive in the human stomach. Hp infection is one of the
important factors of gastric cancer. This bacterial infection can cause chronic gastritis, and lead to gastric ulcer
and gastric atrophy, and even gastric cancer. The purpose of this article is to study the effect of Helicobacter
pylori on the proliferation and apoptosis of gastric epithelial cells and gastric cancer cells, and observe the
human poorly differentiated gastric adenocarcinoma cell line MGC-803, human gastric epithelial cell GES-1,
human gastric cancer cell line through an inverted microscope BGC-823, human gastric adenocarcinoma cell
line SGC-7901 and Hp live bacterial suspension with a multiplicity of infection of 100/1, 200/1 were incubated
for 24 and 48 hours in the experimental group and the control group (without adding Hp live bacterial
suspension) Changes in cell morphology. The results showed that after Hp treatment, the normal morphology of
the four types of cells changed significantly with the extension of culture time and the increase of the number of
bacteria. After treatment with Hp for 24h, the expression levels of 4 kinds of apoptosis and proliferating proteins
were changed in different degrees. After 48 hours of infection with live Hp bacterial suspension, all four types
of cells were inhibited by proliferation activity, and the inhibition rate of cell proliferation activity was 40.1 ±
3.7 under the influence of multiple Hp suspensions of 200/1 infection, which were significantly higher than the
multiple infection. 100 / 1MGC-803 cells (32.7 ± 3.1), GES-1 cells (35.2 ± 1.5), etc. Therefore, Hp can cause
apoptosis and proliferation disorder of gastric cancer cells and normal gastric epithelial cells.

Published
2020-04-01