Microscope Technology in Diagnosis of Tuberculous Meningitis and Detection of Drug Resistance

  • Shihui Liu
Keywords: Tuberculous Meningitis, Drug Resistance, Microscope Observation, Substance Sensitivity Detection Technology, Roche Culture


Tuberculous meningitis is a central infectious disease caused by Mycobacterium tuberculosis. It is the most
serious form of extrapulmonary tuberculosis. It invades the meninges and sometimes involves the parenchyma
and spinal cord. TB is particularly prevalent in developing countries. With the widespread use of anti-TB drugs,
the number of patients with drug-resistant tuberculous meningitis has increased, which has seriously affected the
prognosis of tuberculous meningitis. In the treatment of patients with drug-resistant tuberculous meningitis,
early diagnosis, empirical treatment and adjustment of treatment plan are particularly important for the
prognosis of patients. Based on the above background, the purpose of this article is based on the application of
microscopy in the diagnosis of tuberculous meningitis and the detection of drug resistance. In this paper, 50
patients with clinical diagnosis of TBM were randomly selected, and the cerebrospinal fluid of 50 patients was
cultured with MODS technology. Simultaneously, four anti-tuberculosis drugs were tested for isoniazid,
rifampicin, streptomycin and ethambutol. And compared with traditional Roche culture and Roche drug
sensitivity results. The coincidence rate of positive results with Roche culture was 88%, and the average time of
positive culture results was 8d and 26d, respectively. The detection rates of isoniazid, rifampicin, streptomycin,
ethambutol and Roche were 88%, 96%, 92%, and 84%, respectively. The average time required for drug
sensitivity testing was 8d and 31d, respectively. The experimental results found that the diagnosis of TBM and
drug resistance testing by MODS has the advantages of fast, simple operation and low cost.