Application of Microscope in the Study of Aquatic Environment

  • Guobin Chen, Zengwu Sun, M. M. Kamruzzaman


In order to study the application of microscope in aquatic environment, in this paper, the common live algae, Microcystis aeruginosa was selected as the research object, and the culture was carried out and the live algae was quickly killed by hydroxyl radical method. The morphology, killing threshold and the changes of endophytic and DOC (dissolved organic carbon) concentrations of live algae at this stage was observed, and the effect result was compared. In the algae culture process, it was found that algae grew slowly at the first 5 days of culture, and grew fastest in 5 to 12 days, which was the logarithmic growth phase. And it entered the stable phase after 12 days, and the maximum algal density is 8.50×106 cells/ml. In the morphological analysis of -OH killed algae, it was found that the pleats of the cells increased with the increase of TRO (threshold) concentration, and even broke when the killing threshold was exceeded. It can be found that although the scanning electron micrograph can’t directly distinguish the living algae and dead algae cells, but according to the morphology of most algae cells in different samples, the morphological changes of live algae cells and dead algae cells before and after treatment can be speculated. In the analysis of the threshold of -OH killed microcystis aeruginosa and the change of chlorophyll a and DOC content of algae, it was found that the killing threshold of -OH completely killed algae was 1.37mg/L, and the concentration of chlorophyll decreased with the increase of TRO concentration. The concentration of organic DOC increased with increasing TRO concentration. Therefore, through the research in this paper, it was found that the -OH can quickly kill the common algae in the water bloom and the effect was obvious. It is possible to use -OH as a highly effective and safe oxidant for the elimination of water bloom. Although there are some shortcomings in the research process, it still provides experimental basis for the treatment of the water environment in the later stage, which has important research significance.