Analysis of Effect of Taxus Cuspidata Polysaccharide on Hypoglycemic Effect in Mice

  • Wei Zhao, Yan Shi, Qinyuan Liu

Abstract

To explore the hypoglycemic effect of active polysaccharides from the branches and leaves of Taxus cuspidata on mice, and provide a scientific basis for the further study of the mechanism of hypoglycemic effect of Taxus cuspidata, in this study, the active polysaccharides from the branches and leaves of Taxus cuspidata were extracted and isolated, and the hypoglycemic mechanism of Taxus cuspidata polysaccharides was studied by establishing a mouse model of Type II diabetes. In this study, the polysaccharides in the branches and leaves of Taxus cuspidata were separated and purified to obtain the polysaccharides of Taxus cuspidata. 10 mice were selected as the blank group. 30 mice were screened as grouped subjects in the established type II diabetic mice. They were randomly divided into model group, high dose Taxus polysaccharide (500 mg/kg) group and low dose Taxus polysaccharide group (125 mg/kg). The hypoglycemic activity of Taxus cuspidata in leaves was confirmed by comparison, and the effect of Taxus polysaccharide on pancreatic tissue of mice was analyzed. The hypoglycemic activity of Taxus polysaccharides was further analyzed by establishing an insulin resistance HepG2 cell model. The experimental results show that the polysaccharides of Taxus cuspidata have consumption obvious effects on glucose consumption, which can reduce blood sugar and may be related to the change of sugar metabolism and insulin-like effects of Taxus polysaccharides. It was also found that the polysaccharide of Taxus can protect the pancreatic tissue of model mice while lowering blood sugar. The experiment found that Taxus polysaccharide could promote the glucose consumption of insulin-resistant HepG2 cells, and the best effect was obtained when the administration concentration was 0.05 mg/mL, which was significantly different from the model group (P<0.05). There are relatively few studies on the polysaccharides of Taxus. Therefore, it is necessary to further study its chemical composition, make its chemical composition clear, and at the same time conduct screening test on bioactive constituents, and clarify the mechanism of action to better provide a basis for clinical medicine use.

Published
2019-06-21