Smut of Corn Smut and Protein by Atomic Force Microscopy

  • Decao Ma, Xiaohong Wang, Yuan He, Yan Gao

Abstract

Understanding the surface morphology of cells is an important basis for further understanding of cell function and exploring life phenomena. On the one hand, AFM and optical microscopy were used to characterize the surface morphology of S. cerevisiae cells, and the effects of bacteriostatic agents, wilting and hygromycin on the surface morphology of S. cerevisiae cells were observed. Further analysis explained the mechanism of drug influence on cells; on the other hand, the interaction of bud-coating proteins was studied by AFM single molecular force spectrum. The morphological changes of the growth of the sphaerotheca fuliginous during the haploid period were observed by AFM, and the black powder bacteria in the air for a long time were observed. The morphology of the growth of the haploid tube of the black powder fungus and the surface morphology of the microbial cells of several tens of nanometers can be clearly observed by the AFM image. The effects of bacteriostatic agents, wilting and hygromycin on the cell morphology of S. cerevisiae were studied by AFM. The results showed that the rust-free spirit not only inhibited the growth of the black powder fungus, but also appeared many small pits on the surface of the black powder fungus.

Published
2019-06-21