Application Technology of Electrochemical Sensors Based on New Nanomaterials

  • Shaojun Zhang, Mingyu Wang, Tianshu Wang

Abstract

In order to study the application technology of electrochemical sensors based on new nanomaterials, AuNPs nanomaterials were prepared, and then Nafion-Ru (bpy)32+-AuNPs complex solution was obtained by electrostatic interaction. The electrochemical immunosensor was prepared by electrochemiluminescence method, and its performance was analyzed by TTX (Tetrodotoxin) measurement. It was found that the new nano-materials were characterized by clear bright spots and successfully adhered. The electrochemical behavior of ECL (electro-chemiluminescent) immunosensor was analyzed. It was found that with the gradual modification of the materials, the impedance value gradually increased. The results showed that the biosensor was successfully constructed; in the optimization analysis of ECL experimental conditions, it was found that the optimum experimental conditions were PBS (Phosphate Buffered Saline) with pH of 7.5, incubation time of 60 minutes and deposition voltage of 0.6V; in TTX detection analysis, it was found that the signal of electro-chemiluminescent sensor tended to be stable after a certain scanning time, which indicated that the immunosensor was very stable, and suitable for subsequent TTX detection; in sample analysis, it was found that the RSD (relative standard deviation) was 3.4-8.1% and the recovery was 98.0-104.2% by adding different concentrations of TTX to standard samples. The experiments show that through the research on the application technology of the new nano-material electrochemical sensor, a label-free self-enhanced electrochemiluminescence immunosensor is prepared, which has good conductivity and sensitivity. Therefore, the immunosensor has good practical value. Although there are some shortcomings in the research process, it still provides a reference for the production of bio-immune labeling sensor in the future.

Published
2019-05-19